An integrated information management model for proactive prevention of struck-by-falling-object accidents on construction sites

The construction industry is one of the most hazardous industries in many countries. Struck-by-falling-object accidents are not given adequate attention even though they are of great importance on construction sites. The objective of this paper is to analyze the proactively preventive information requirement of struck-by-falling-object accidents and propose an integrated information management model using a ZigBee RFID sensor network to fulfill these requirements. First, the frequency of particular type of objects is analyzed based on vast historical accident cases. Next, this paper analyzes the proactively preventive information requirement of struck-by-falling-object accidents and brought forth an integrated information management model. Furthermore, considering the crucial and controllable objects, an integrated information management model is proposed. This study would provide a possible approach for tracking struck-by-falling-object accidents based on real-time information for proactive prevention and could serve as a foundation for further study. © 2012 Elsevier B.V. All rights reserved

Early Mesozoic tectonic transition of the eastern South China Block: constraints from Late Triassic Dashuang complex in eastern Zhejiang Province

<p>The Mesozoic tectonic transition from the Palaeo-Tethys tectonic regime to the Palaeo-Pacific tectonic regime in the eastern South China Block has long been debated. Geochemical and zircon U–Pb–Hf isotopic studies were conducted on the Dashuang complex in the eastern Zhejiang Province. The Dashuang complex consists mainly of quartz syenite in the northwestern part and quartz monzonite in the southeastern part. New laser ablation inductively coupled plasma mass spectrometry zircon U–Pb data show that the quartz syenite, the quartz monzonite, and its chilled margin (fine-grained granite) crystallized at 235 ± 4 Ma, 232 ± 3 Ma, and 230 ± 1 Ma, respectively. The Dashuang complex intrudes into the Chencai Group gneiss that postdated ~646 Ma and underwent anatexis at 443 ± 14 Ma. The quartz monzonite shows A-type granite affinity, characterized by high K₂O + Na₂O and Zr + Nb + Ce + Y, high FeO_T/(MgO + FeO_T) and Ga/Al ratios, an enrichment in light rare earth elements, and depletions in Ba, Sr, and Eu. The quartz monzonite has zircon ε_Hf(<i>t</i>) values of −14.2 to –11.9 and two-stage model ages of 1788–1922 Ma. Zircon ε_Hf(<i>t</i>) values of the chilled margin (fine-grained granite) and wall rock (gneiss) are scattered (−18.2 to –6.3 and −19.5 to 10.2). The corresponding two-stage model ages are 1482–2133 Ma and 1184–2471 Ma, respectively. The Dashuang complex was derived mainly from partial melting of Neoproterozoic clastic rocks in the Cathaysia Block. Geochemical data indicate that the quartz monzonite formed in a post-collision extensional environment. These results, considered with previous data, indicate that the transition from the Palaeo-Tethys to the Palaeo-Pacific tectonic regimes of the eastern South China Block occurred during the Late Triassic (225–215 Ma).</p

Design and implementation of an identification system in construction site safety for proactive accident prevention

Identifying accident precursors using real-time identity information has great potential to improve safety performance in construction industry, which is still suffering from day to day records of accident fatality and injury. Based on the requirements analysis for identifying precursor and the discussion of enabling technology solutions for acquiring and sharing real-time automatic identification information on construction site, this paper proposes an identification system design for proactive accident prevention to improve construction site safety. Firstly, a case study is conducted to analyze the automatic identification requirements for identifying accident precursors in construction site. Results show that it mainly consists of three aspects, namely access control, training and inspection information and operation authority. The system is then designed to fulfill these requirements based on ZigBee enabled wireless sensor network (WSN), radio frequency identification (RFID) technology and an integrated ZigBee RFID sensor network structure. At the same time, an information database is also designed and implemented, which includes 15 tables, 54 queries and several reports and forms. In the end, a demonstration system based on the proposed system design is developed as a proof of concept prototype. The contributions of this study include the requirement analysis and technical design of a real-time identity information tracking solution for proactive accident prevention on construction sites. The technical solution proposed in this paper has a significant importance in improving safety performance on construction sites. Moreover, this study can serve as a reference design for future system integrations where more functions, such as environment monitoring and location tracking, can be added

Characterization of a putative ArsR transcriptional regulator encoded by <i>Rv2642</i> from <i>Mycobacterium tuberculosis</i>

Characterization of a putative ArsR transcriptional regulator encoded by <i>Rv2642</i> from <i>Mycobacterium tuberculosis</i

Proteome-wide Lysine Glutarylation Profiling of the <i>Mycobacterium tuberculosis</i> H37Rv

Lysine glutarylation, a new protein posttranslational modification (PTM), was recently identified and characterized in both prokaryotic and eukaryotic cells. To explore the distribution of lysine glutarylation in <i>Mycobacterium tuberculsosis</i>, by using a comprehensive method combining the immune affinity peptide enrichment by the glutaryl-lysine antibody with LC–MS, we finally identified 41 glutarylation sites in 24 glutarylated proteins from <i>M. tuberculosis</i>. These glutarylated proteins are involved in various cellular functions such as translation and metabolism and exhibit diverse subcellular localizations. Three common glutarylated proteins including 50S ribosomal protein L7/L12, elongation factor Tu, and dihydrolipoamide succinyltransferase are shared between <i>Escherichia coli</i> and <i>M. tuberculosis</i>. Moreover, comparison with other PTMs characterized in <i>M. tuberculosis</i>, 15 glutarylated proteins, are found to be both acetylated and succinylated. Notably, several stress-response-associated proteins including HspX are glutarylated. Our data provide the first analysis of <i>M. tuberculosis</i> lysine glutarylated proteins. Further studies on the role of the glutarylated proteins will unveil the molecular mechanisms of glutarylation underlying <i>M. tuberculosis</i> physiology and pathogenesis

Proteome-wide Lysine Glutarylation Profiling of the <i>Mycobacterium tuberculosis</i> H37Rv

Lysine glutarylation, a new protein posttranslational modification (PTM), was recently identified and characterized in both prokaryotic and eukaryotic cells. To explore the distribution of lysine glutarylation in <i>Mycobacterium tuberculsosis</i>, by using a comprehensive method combining the immune affinity peptide enrichment by the glutaryl-lysine antibody with LC–MS, we finally identified 41 glutarylation sites in 24 glutarylated proteins from <i>M. tuberculosis</i>. These glutarylated proteins are involved in various cellular functions such as translation and metabolism and exhibit diverse subcellular localizations. Three common glutarylated proteins including 50S ribosomal protein L7/L12, elongation factor Tu, and dihydrolipoamide succinyltransferase are shared between <i>Escherichia coli</i> and <i>M. tuberculosis</i>. Moreover, comparison with other PTMs characterized in <i>M. tuberculosis</i>, 15 glutarylated proteins, are found to be both acetylated and succinylated. Notably, several stress-response-associated proteins including HspX are glutarylated. Our data provide the first analysis of <i>M. tuberculosis</i> lysine glutarylated proteins. Further studies on the role of the glutarylated proteins will unveil the molecular mechanisms of glutarylation underlying <i>M. tuberculosis</i> physiology and pathogenesis

Gallium Nitride Based Logpile Photonic Crystals

We demonstrate a nine-layer logpile three-dimensional photonic crystal (3DPC) composed of single crystalline gallium nitride (GaN) nanorods, ∼100 nm in size with lattice constants of 260, 280, and 300 nm with photonic band gap in the visible region. This unique GaN structure is created through a combined approach of a layer-by-layer template fabrication technique and selective metal organic chemical vapor deposition (MOCVD). These GaN 3DPC exhibit a stacking direction band gap characterized by strong optical reflectance between 380 and 500 nm. By introducing a “line-defect” cavity in the fifth (middle) layer of the 3DPC, a localized transmission mode with a quality factor of 25–30 is also observed within the photonic band gap. The realization of a group III nitride 3DPC with uniform features and a band gap at wavelengths in the visible region is an important step toward realizing complete control of the electromagnetic environment for group III nitride based optoelectronic devices

Atom Probe Tomography of <i>a</i>-Axis GaN Nanowires: Analysis of Nonstoichiometric Evaporation Behavior

GaN nanowires oriented along the nonpolar <i>a</i>-axis were analyzed using pulsed laser atom probe tomography (APT). Stoichiometric mass spectra were achieved by optimizing the temperature, applied dc voltage, and laser pulse energy. Local variations in the measured stoichiometry were observed and correlated with facet polarity using scanning electron microscopy. Fewer N atoms were detected from nonpolar and Ga-polar surfaces due to uncorrelated evaporation of N₂ ions following N adatom diffusion. The observed differences in Ga and N ion evaporation behaviors are considered in detail to understand the influence of intrinsic materials characteristics on the reliability of atom probe tomography analysis. We find that while reliable analysis of III–N alloys is possible, the standard APT procedure of empirically adjusting analysis conditions to obtain stoichiometric detection of Ga and N is not necessarily the best approach for this materials system

First Succinyl-Proteome Profiling of Extensively Drug-Resistant <i>Mycobacterium tuberculosis</i> Revealed Involvement of Succinylation in Cellular Physiology

Protein lysine succinylation, an emerging protein post-translational modification widespread among eukaryotic and prokaryotic cells, represents an important regulator of cellular processes. However, the extent and function of lysine succinylation in <i>Mycobacterium tuberculosis</i>, especially extensively drug-resistant strain, remain elusive. Combining protein/peptide prefractionation, immunoaffinity enrichment, and LC–MS/MS analysis, a total of 686 succinylated proteins and 1739 succinylation sites of <i>M. tuberculosis</i> were identified, representing the first global profiling of <i>M. tuberculosis</i> lysine succinylation. The identified succinylated proteins are involved in a variety of cellular functions such as metabolic processes, transcription, translation, and stress responses and exhibit different subcellular localization via GO, protein interaction network, and other bioinformatic analysis. Notably, proteins involved in protein biosynthesis and carbon metabolism are preferred targets of lysine succinylation. Moreover, two prevalent sequence patterns: EK^suc and K*****K^suc, can be found around the succinylation sites. There are 109 lysine-succinylated homologues in <i>E. coli</i>, suggesting highly conserved succinylated proteins. Succinylation was found to occur at the active sites predicted by Prosite signature including Rv0946c, indicating that lysine succinylation may affect their activities. There is extensive overlapping between acetylation sites and succinylation sites in <i>M. tuberculosis</i>. Many <i>M. tuberculosis</i> metabolic enzymes and antibiotic resistance proteins were succinylated. This study provides a basis for further characterization of the pathophysiological role of lysine succinylation in <i>M. tuberculosis</i>

First Succinyl-Proteome Profiling of Extensively Drug-Resistant <i>Mycobacterium tuberculosis</i> Revealed Involvement of Succinylation in Cellular Physiology

Protein lysine succinylation, an emerging protein post-translational modification widespread among eukaryotic and prokaryotic cells, represents an important regulator of cellular processes. However, the extent and function of lysine succinylation in <i>Mycobacterium tuberculosis</i>, especially extensively drug-resistant strain, remain elusive. Combining protein/peptide prefractionation, immunoaffinity enrichment, and LC–MS/MS analysis, a total of 686 succinylated proteins and 1739 succinylation sites of <i>M. tuberculosis</i> were identified, representing the first global profiling of <i>M. tuberculosis</i> lysine succinylation. The identified succinylated proteins are involved in a variety of cellular functions such as metabolic processes, transcription, translation, and stress responses and exhibit different subcellular localization via GO, protein interaction network, and other bioinformatic analysis. Notably, proteins involved in protein biosynthesis and carbon metabolism are preferred targets of lysine succinylation. Moreover, two prevalent sequence patterns: EK^suc and K*****K^suc, can be found around the succinylation sites. There are 109 lysine-succinylated homologues in <i>E. coli</i>, suggesting highly conserved succinylated proteins. Succinylation was found to occur at the active sites predicted by Prosite signature including Rv0946c, indicating that lysine succinylation may affect their activities. There is extensive overlapping between acetylation sites and succinylation sites in <i>M. tuberculosis</i>. Many <i>M. tuberculosis</i> metabolic enzymes and antibiotic resistance proteins were succinylated. This study provides a basis for further characterization of the pathophysiological role of lysine succinylation in <i>M. tuberculosis</i>